Purpose
We are seeking a technically critical, independent evaluation of DotMatch 0.2.1 for CRISPR guide counting. The aim is to test whether its input contract, count outputs, and explicit unique, ambiguous, none, and invalid states fit an existing research workflow. This is a request for comparison and fault-finding, not an endorsement.
Suitable evaluation
- use a public, synthetic, or otherwise shareable FASTQ fixture and guide library
- install
dotmatch==0.2.1 from PyPI or Bioconda
- compare the count output with the workflow currently used for the same input
- inspect
sample_qc.tsv, summary.json, assignments.tsv, and top_unmatched.tsv where applicable
- report command lines, package versions, target-window assumptions, disagreements, and blockers
The documented protocol is at https://github.com/dnncha/dotmatch/blob/main/docs/pilot-program.md. The CRISPR first-run tutorial is at https://dotmatch.readthedocs.io/en/latest/tutorials/crispr-count-first-run.html.
Do not upload private sequencing data, patient data, restricted assay designs, or confidential screenshots. A minimized reproduction or public accession is sufficient.
Completion
This issue can close after one independent evaluator records a reproducible comparison or explains why the current command or output contract does not fit their workflow. Any project or organization name used in a separate public record requires explicit approval from that party.
Generated by Codex
Purpose
We are seeking a technically critical, independent evaluation of DotMatch 0.2.1 for CRISPR guide counting. The aim is to test whether its input contract, count outputs, and explicit
unique,ambiguous,none, andinvalidstates fit an existing research workflow. This is a request for comparison and fault-finding, not an endorsement.Suitable evaluation
dotmatch==0.2.1from PyPI or Biocondasample_qc.tsv,summary.json,assignments.tsv, andtop_unmatched.tsvwhere applicableThe documented protocol is at https://github.com/dnncha/dotmatch/blob/main/docs/pilot-program.md. The CRISPR first-run tutorial is at https://dotmatch.readthedocs.io/en/latest/tutorials/crispr-count-first-run.html.
Do not upload private sequencing data, patient data, restricted assay designs, or confidential screenshots. A minimized reproduction or public accession is sufficient.
Completion
This issue can close after one independent evaluator records a reproducible comparison or explains why the current command or output contract does not fit their workflow. Any project or organization name used in a separate public record requires explicit approval from that party.
Generated by Codex