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Thomas-Otavio Peulen edited this page Aug 4, 2019 · 5 revisions

Below you find video tutorials for ChiSurf for the following use-cases

1. Introduction

ChiSurf tutorial 1.01: Overview, loading data, creating fits

  • Loading files
  • Selecting a model
  • Creating a fit

ChiSurf tutorial 1.02: Analysis of fluorescence decays

  • TCSPC data formats
  • Load an example data set
  • Correction parameters
  • Analysis by multi exponential decay
  • Save the analysis

2. Global fitting of

  • (D0)_(DexDem): a donor emission of a donor reference samples (D0) excited by a "donor" laser (Dex) and detected in a donor channel (Dem)
  • (DA)_(DexDem): the donor emission of a FRET sample (DA) excited by a "donor" laser (Dex) and detected in the "donor" detection channel (Dem)
  • (DA)_(AexAem): the acceptor emission of FRET sample (DA) excited by an "acceptor" laser (Aex) and detected in the "acceptor" detection channel (Aem)
  • (DA)_(DexAem): the acceptor emission of FRET sample (DA) excited by a "donor" laser (Dex) and detected in the "acceptor" detection channel (Aem)

ChiSurf tutorial: FRET sensitized emission

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